Kamentsky L, Jones TR, Fraser A, Bray M-A, Logan DJ, Madden KL, et al. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. doi: 10.1016/j.jbiotec.2017.07.028Ĭarpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, et al. KNIME for reproducible cross-domain analysis of life science data. ImageJ2: ImageJ for the next generation of scientific image data. Rueden CT, Schindelin J, Hiner MC, DeZonia BE, Walter AE, Arena ET, et al. 3D, three-dimensional FISH, fluorescent in situ hybridization GAPDH, glyceraldehyde 3-phosphate dehydrogenase WGA, wheat germ agglutinin.Įliceiri KW, Berthold MR, Goldberg IG, Ibáñez L, Manjunath BS, Martone ME, et al. Images were provided by Javier Frias Aldeguer and Nicolas Rivron from Hubrecht Institute, Netherlands, and are available from the Broad Bioimage Benchmark Collection ( ). The underlying measurements may be downloaded as S1 File. (F) Examples of analysis that can be done by CellProfiler: (top) cell volume relative nucleus volume, (middle) GAPDH transcript quantity in each cell using CellProfiler’s “RelateObjects” module, (bottom) number of GAPDH transcripts in Z-plane (bin size = 2.5 μm). (E) Segmentation of GAPDH transcript foci using CellProfiler, as viewed in Fiji. (D) Segmentation of cells after setting nuclei as seeds by CellProfiler, as viewed in Fiji. (C) Nuclei after segmentation by CellProfiler, as viewed in Fiji. Figure labels: RH (“RemoveHoles”), Close (“Closing”), Erode (“Erosion”), Mask (“MaskImage”), Math (“ImageMath”), EorS Features (“EnhanceOrSuppressFeatures”). (B) CellProfiler 3.0 image processing modules used for membrane image processing. (A) Original 3D image of blastocyst cell membrane prior to analysis. Rangers Centre 2.24km Andrew Jackson Centre 2.24km Ulster Folk and Transport Museum 6.82km Train Station Carrickfergus Railway Station 0.68km Clipperstown Railway Station 0.72km Downshire Railway Station 1.77km Trooperslane Railway Station 2.40km Greenisland Railway Station 4.12km Jordanstown Railway Station 5.99km Helen's Bay Railway Station 6.79km Whiteabbey Railway Station 7.32km Whitehead Railway Station 8.03km Carnalea Railway Station 8.49km Mossley West Railway Station 9.08km Golf Course Carrickfergus Golf Club 1.23km Greenisland Golf Club 4.25km Royal Belfast Golf Club 5.98km Bentra Golf Course 7.92km Blackwood Golf Centre 10.68km College Carrickfergus College 1.78km Ulidia Integrated College 2.40km Cricket Field Carrickfergus Cricket Club Ground 2.78km Greenisland Cricket Ground 4.54km Hill Slieve True 6.66km Park Crawfordsburn Country Park 7.44km Redburn Country Park 9.70km Golf Driving Range Whitehead Driving Range 7.86km Bar Holywood Maypole 8.16km Heritage Railway Steam Train Rides 8.22km Conservation Area Diamond Jubilee Wood 8.25km Waterfall Gleno Waterfall 9.92km Zoo Belfast Zoo 10.37km Suburb Bellevue 10.43km Shop Logwood Plant Centre 10.50km Church St Gerard's Church 10.64km Garden Sunken Gardens 10.Images were captured of a mouse embryo blastocyst cell membrane stained with WGA and FISH for GAPDH transcripts. What's nearby Marina Carrickfergus Marina 0.13km Bangor Marina 10.43km Museum Flame The Gasworks Museum of Ireland 0.34km Carrickfergus Museum and Civic Centre 0.65km U.S.
0 Comments
Leave a Reply. |